Journal: Chinese Medical Journal

Article Title: Suppression of METTL3 expression attenuated matrix stiffness-induced vaginal fibroblast-to-myofibroblast differentiation and abnormal modulation of the extracellular matrix in pelvic organ prolapse

doi: 10.1097/cm9.0000000000003409

Figure Lengend Snippet: Figure 2: (A–E) Representative gene expression of vaginal fibroblasts cultured on PA gels with variable stiffness (0.2 kPa vs. 12 kPa). WB analysis of protein expression and RT–qPCR analysis of the mRNA expression of α-SMA (A), COL1A1 (B), TIMP1 (C), TIMP2 (D), COL1A1/COL3A1 (E). n = 5; (F) EdU assays comparing vaginal fibroblast proliferation between 0.2 kPa and 12 kPa. (G) Representative images (IF) showing the effect of matrix stiffness on α-SMA, a marker of fibroblast-to-myofibroblast differentiation. GAPDH was used as an internal control. Scale bar = 100 µm. *P <0.05, †P <0.01, ‡P <0.001. α-SMA: α-smooth muscle actin; COL1A1: Collagen Type 1 Alpha 1; COL3A1: Collagen Type 3 Alpha 1; DAPI: 4′,6-diamidino-2-phenylindole; GAPDH: Glyceraldehyde-phosphate dehydroge nase; IF: Immunofluorescence; PA: Polyacrylamide; RT–qPCR: Real-time polymerase chain reaction; TIMP1: Tissue inhibitor of matrix metalloproteinase 1; TIMP2: Tissue inhibitor of matrix metalloproteinase 2; WB: Western blot.

Article Snippet: The membranes were blocked for 30 min and incubated with the following antibodies overnight at 4°C: anti-METTL3 (1:1000; rabbit polyclonal antibody, ABclonal; Wuhan, China); anti-α-smooth muscle actin (α-SMA) (1:1000; rabbit monoclonal antibody, ABclonal); anti-collagen Type I Alpha 1 (COL1A1) (1:800; rabbit polyclonal antibody, ABclonal); anti-collagen Type 3 Alpha 1 (COL3A1) (1:800; rabbit monoclonal antibody, ABclonal); anti-tissue inhibitor of matrix metalloproteinase 1 (TIMP1) (1:1000; rabbit polyclonal antibody, ABclonal); anti-tissue inhibitor of matrix metalloproteinase 2 (TIMP2) (1:1000; Proteintech, rabbit polyclonal antibody, Wuhan, China); anti-glyceraldehyde-phosphate dehydrogenase (GAPDH) (1:1000; rabbit monoclonal antibody, ABclonal).

Techniques: Gene Expression, Cell Culture, Expressing, Quantitative RT-PCR, Marker, Control, Immunofluorescence, Real-time Polymerase Chain Reaction, Western Blot

Journal: Drug Design, Development and Therapy

Article Title: Protective effect of fucoidan from Fucus vesiculosus on liver fibrosis via the TGF-β1/Smad pathway-mediated inhibition of extracellular matrix and autophagy

doi: 10.2147/dddt.s98740

Figure Lengend Snippet: Figure 2 Effect of fucoidan on ECM in liver fibrosis. Notes: (A) Fucoidan decreased the level of HA and LN. (B) The analysis of Western blotting showed that fucoidan obviously changed the expression of collagen I, α-SMA, MMP-9, and TIMP1. (C) The mRNA levels of collagen 1α1, α-SMA were significantly downregulated by fucoidan. Data are expressed as mean ± SD (n=8, *P,0.05 for CCl4 [BDL] versus control, #P,0.05 for CCl4 [BDL] + fucoidan versus CCl4 [BDL], ^P,0.05 for CCl4 [BDL] + fucoidan [25 mg/kg] versus CCl4 [BDL] + fucoidan [10 mg/kg],

Article Snippet: The primary antibodies used were as follows: α-SMA, TIMP1, MMP-9, Co-I, Beclin-1, P62 (1:500, all from Proteintech Group, Inc., Chicago, IL, USA); TGF-β1, smad2, p-smad2, LC3, β-actin (1:1,000, all from Cell Signaling Technology, Danvers, MA, USA); and smad3, p-smad3 (1:500, both from ABclonal, Cambridge, MA, USA).

Techniques: Western Blot, Expressing, Control

Journal: Antioxidants

Article Title: Antioxidant, Anti-Inflammation and Antiaging Activities of Artocarpus altilis Methanolic Extract on Urban Particulate Matter-Induced HaCaT Keratinocytes Damage

doi: 10.3390/antiox11112304

Figure Lengend Snippet: Effect of AAM on PM-induced aging protein expressions in HaCaT cells. MMP-1 and MMP-2 ( A ), TIMP1 ( B ). Data were from three independent experiments. Value are mean ± SD ( n = 3). # Significantly different from control group ( p < 0.05); * Significantly different from PM treatment alone ( p < 0.05).

Article Snippet: After blocking for 1 h, the membranes were incubated overnight with primary antibodies, including AQP3, TNF-α (1:1000 dilution, ABclonal, Woburn, MA, USA), COX-2, MMP-2, TIMP1, phospho-SAPK/JNK (1:1000 dilution, Cell Signaling Technology, Danvers, MA, USA), 4HNE, p-p38α (1:1000, Merck Millipore Corporation, Billerica, MA, USA), p-ERK (1:2500, Merck Millipore Corporation, Billerica, MA, USA), Loricrin, MMP-1 (1:1000, Proteintech, Chicago, IL, USA), Involucrin (1:1000, Arigo Biolaboratories, Hsinchu, Taiwan, China), filaggrin (1:500, Santa Cruz Biotechnology, Dallas, TX, USA) and GAPDH (1:2500, Santa Cruz Biotechnology, Dallas, TX, USA) at 4 °C.

Techniques: Control

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Gene sequence

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Sequencing

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Collagen-modified factors detection ( a ) Quantitative real-time PCR analysis of Col-I and Col-III mRNA expression of hPDLCs. n = 3 . ( b ) Western blot analysis of Col-I and Col-III protein levels of hPDLCs. n = 3 . Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. the control group, #P < 0.05 vs. the tensile stress group and &P < 0.05 vs. the laser group by one-way ANOVA

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Modification, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Gene sequence

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Sequencing

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Osteogenic differentiated factors and Osteoclast differentiated factors detection ( a ) Quantitative real-time PCR analysis of Runx2 and OCN mRNA expression of hPDLCs. n = 3 . ( b ) Western blot analysis of Runx2 and OCN protein levels of hPDLCs. n = 3 . ( c ) Quantitative real-time PCR analysis of NFATC1, c-Fos and CTSK mRNA expression of hPDLCs. n = 3 . ( d ) Western blot analysis of NFATC1, C-FOS and CTSK protein levels of hPDLCs. n = 3 . Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. the control group, #P < 0.05 vs. the tensile stress group and &P < 0.05 vs. the laser group by one-way ANOVA

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Gene sequence

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Sequencing

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Gene sequence

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Sequencing

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Osteogenic differentiated factors and Osteoclast differentiated factors detection ( a ) Quantitative real-time PCR analysis of Runx2 and OCN mRNA expression of hPDLCs. n = 3 . ( b ) Western blot analysis of Runx2 and OCN protein levels of hPDLCs. n = 3 . ( c ) Quantitative real-time PCR analysis of NFATC1, c-Fos and CTSK mRNA expression of hPDLCs. n = 3 . ( d ) Western blot analysis of NFATC1, C-FOS and CTSK protein levels of hPDLCs. n = 3 . Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. the control group, #P < 0.05 vs. the tensile stress group and &P < 0.05 vs. the laser group by one-way ANOVA

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control